Abstract
A chemical investigation on the cultures extract of the basidiomycete Armillaria gallica Marxm. & Romagn. led to the isolation of eight compounds, including a new steroid armillanal D (1), a new natural product VD2-1 (8) and six known compounds. The structure of 1 was elucidated on the basis of extensive 1D and 2D-NMR spectra analysis, HR-ESI-MS, and electronic circular dichroism (ECD) spectra. The inhibitory activity of these compounds except 8 on NLRP3 inflammasome activation in Nigericin-induced mouse macrophage (J774A.1) cells was evaluated. Chaxine C (6) exhibited potential antiinflammatory activity in vitro with IC50 value of (4.97 +/- 0.81) mu mol/L.