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Comparison of drying methods in the fabrication of collagen scaffold via indirect rapid prototyping
Journal article   Peer reviewed

Comparison of drying methods in the fabrication of collagen scaffold via indirect rapid prototyping

Wai-Yee Yeong, Chee-Kai Chua, Kah-Fai Leong, Margam Chandrasekaran and Mun-Wai Lee
Journal of biomedical materials research. Part B, Applied biomaterials, Vol.82(1), pp.260-266
07/2007
PMID: 17183564

Abstract

Animals Cattle Collagen Type I - ultrastructure Freeze Drying Porosity Preservation, Biological - methods Tissue Engineering
Porous collagen scaffolds with predefined 3-dimesional (3-D) networks of internal channels are fabricated via an indirect rapid prototyping technique. To obtain the scaffolds, two drying methods, namely critical point drying and freeze-drying were investigated. The latter was found to be a more suitable process as it induced less shrinkage and reproduced the design morphology accurately. The resulting scaffold contained internal hollow channels with porous foam-like structure occupying the surrounding volume. The mean diameter of the pores was 180 +/- 60 microm and the channels diameter obtained was measured as 437 +/- 100 microm. The channels were defined and strengthened by a layer of skin due to the process of freeze-drying. These networks of internal channels serve to enhance the mass transport rate through the scaffold and help to increase the depth of cells penetration in the scaffold.

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