Abstract
We present an on-demand single cell imaging technique activated by differential resistive pulse sensing in a portable system integrating a microfluidic differential coulter counter and a lensless complementary metal-oxide-semiconductor (CMOS) imaging sensor. Dual parametric single cell analysis and on-demand single cell imaging have been demonstrated by microbeads of different sizes and a cell mixture including red blood cells (RBCs) and tumor cell line HepG2 Cells. The on-demand imaging capability could avoid generating useless images without cells and Tenable selective imaging of single cells within a specific size range.