Abstract
Moderate amounts of ferrous ion (Fe2+) are necessary, and insufficient or excessive amounts can have adverse health effects. Dysregulation of intracellular Fe2+ levels triggers ferroptosis, an iron-mediated programmed cell death associated with a diverse range of diseases. Iron transporters in the Golgi apparatus (GA) affect the Fe2+ level within the cell, thereby affecting the process of ferroptosis. The regulation of ferroptosis holds immense significance in the treatment of various pathological conditions. Therefore, the monitoring of Fe2+ in the GA is imperative. Herein, a novel highly specific GA targeting fluorescent probe (GA-Fe) for Fe2+ was developed using a carbamoyl oxime structure as the recognition group. GA-Fe has good spectral performance and high sensitivity (LOD = 64 nM) for detecting Fe2+. Additionally, GA-Fe exhibits excellent targeting property and can serve as a highly effective tool for labeling the GA. Notably, GA-Fe can not only detect native and exogenous Fe2+ in cells and zebrafish, but also track Fe2+ fluctuations in ferroptosis model.
[Display omitted]
•Probe GA-Fe can quantitatively and sensitively detect Fe2+ with high specifity.•Probe GA-Fe performs the naked-eye detection of Fe2+.•probe GA-Fe was successfully targeted to the Golgi apparatus.•Probe GA-Fe was successfully applied to detect Fe2+ in living cells and zebrafish.•Probe GA-Fe was able to track changes in Fe2+ during ferroptosis.